1. Before you Begin Materials Required but Not Provided • Fresh, anhydrous. Prepare your i3/i3x instrument for shipment. #R7159) APPLICATION NOTE 如何使用SpectraMax M5 多功能微孔. 72 MB. Instrument. FLIPR Calcium 6 and 6-QF Assay Kits provide the largest signal window. Wavelength Range. Fluorescence Microplate Reader Comparison Chart. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. 7. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. SpectraMax M5 microplate reader. Our absorbance plate readers feature our PathCheck Sensor. 0 out of 5 $ 5,999. View and Download Molecular Devices SpectraMax Plus 384 user manual online. 4. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. SpectraMax® M5e. Molecular Devices. 0200-0729. If no sound is heard during initialization, contact technical support. Sensitive fluorescent quantitation of DNA with the Quant-iT. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. 306 plate air cal fail,low light. Optimal instrument. Published Dec 16, 2020 | Updated Dec 16, 2020. 3: The cuvette chamber. The lower limit of detection (LLD) was defined as the level of luciferase detectable above 3 positive standard deviations of the blank. Both microplate and cuvette ports can be qualified. 3 mm. Feb 22, 2023Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. Table1-1 SpectraMax ® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5 e Absorbance X X X X Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. HEALTH AND SAFETY CONSIDERAIONSThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. 7°C and the bottom to 36. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Cavicide (Warehouse, Cat # 79300360) 6. To evaluate the amount of miR-148b mimic released by the retro Diels-Alder reaction (Fig. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Delivers single mode reader performance in one multimode reader package. Other technologies such as imaging. SpectraMax® iD5. The SpectraMax M5 Multi-Mode Microplate Reader measures UV and visible absorbance, fluorescence, luminescence, TRF and HTRF. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. SoftMax Pro Software can collect data from one or more microplates orSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. In . Cables. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. If no values are present, the water constant values are missing from the NVRAM. If you do not have an iLab account you must first make one: this application note, we demonstrate how Molecular Devices microplate readers with luminescence detection mode provide superior sensitivity and ease of use for reliable mycoplasma detection using MycoAlert assays. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax platform. 2. 72 MB. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. SpectraMax ® M5 Multi-Mode Microplate Reader (Molecular Devices) Gemini EM Microplate Reader (Molecular Devices) Methods Cell preparation and analysis. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. performs within its specifications. Configurable readers with triple-mode cuvette ports, validation tools, and compliance softwareProducts : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e reader. Choose cables that meet the requirement described in this appendix. begin reading microplates with seven mouse clicks. Home Resources Videos & Webinars SpectraMax M5e Multi-Mode Microplate Reader The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. The inset, using the same data set, highlights the sensitivity of the assay at low levels of H 2O 2. and California) iv SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. We would like to show you a description here but the site won’t allow us. 315 can't find zero order. Simple, homogeneous workflow minimizes cell handling. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. FlexStation™ I. Pick the best speed, sensitivity, and price for your needs. #I2511) • Assay diluent – may be cell culture mediumThis process should take about 3 - 5 minutes. Category 2 and 3 microplate readers use gradient heating as well. IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175)SpectraMax ® M5 Multi-Mode Microplate Reader (Molecular Devices) Gemini EM Microplate Reader (Molecular Devices) Methods Cell preparation and analysis. AquaMax® 4000. Cavicide (Warehouse, Cat # 79300360) 6. Cells were treated with increasing concentrations of the antagonist atropine and assayed for response to addition of an EC80 concentration of the agonist carbachol. 11/22 2295B. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. SpectraMax M5, Analyst HT or Analyst GT. Step 4: Incubate at room temperature for 1 hour, protected from light. Was the failure only of the Detection Estimate (Mag3) Test for the. For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way?SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. edu) to be added as a trained user in iLab. Plate stacker and robot integration. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. What is fluorescence? Fluorescence is the property of some atoms and molecules to absorb light at a particular wavelength (the excitation: Ex) followed by a short-lived emission (Em) of light at a longer wavelength (Figure 2). Failed to load PDF file. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance X X X X on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. Future ready The SpectraMax i3 x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. An assay plate containing a 15-point standard curve was read on the SpectraMax. 1. The SpectraMax® M3 Multi-Mode Microplate Reader provides three modes of detection in one platform. All results should now be displayed. Table of Contents. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. More Product Information. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The SpectraMax 190 reader can be integrated with Molecular . SoftMax Pro Software can collect data from one or more microplates orSpectraMax ABS Plus SpectraTest ABS2 v1. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. Table 2. Setup recommendations for SpectraMax® M5e. e. moleculardevicescom Molecular Devices C All rigts reserved SpectraMax Quant dsDNA Assay Kits Optimized DNA measurement for your microplate reader assay DATA SHEET Quantitation of DNA is a critical step in molecular biology requiring accuracy, reliability, and the use of increasingly smaller sample volumes for applications such as next-BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 4. ©2022 Molecular Devices, LLC. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. – ®SpectraMax M4 and M5 Multi-Mode Microplate Readers (cat. STAKMAX. View the Molecular Devices website. To update the driver. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. Agilent VWorks Automation Control soft-Only Molecular Devices microplate plate readers offer the capability to . 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. system setup and calibration are controlled from within softmax ro psoftware. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. ©2022 Molecular Devices - All rights. Products : SpectraMax M5/M5e reader Fluorescence-based mitochondrial function analysis (PDF) Summary: Mitochondrial dysfunction is implicated in numerous disease states and is also a major mechanism of drug-induced toxicity. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 01) + 0. ] Example of document to evaluate if the reader . 5. , October 25, 2004 - Molecular Devices Corporation (Nasdaq: MDCC), today announced the introduction of SpectraMax® M5, a five-mode. With optimized reagents, option to operate the readers. Recertification is recommended every two years. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. Non-transfected HEK cells were included as controls. Molecular Devices. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. minutes and begin reading microplates with seven mouse clicks. The single mode Luminescence Readers (LMax II, SpectraMax L), with a Lower Limits of Detection (LLD) use six different light intensities. 4. #6590-0137) • SpectraMax i3x Multi-Mode Microplate Reader • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Specifications subject to change without notice. D. #14-276) 。FAM标记多肽底物 (Molecular Devices Cat. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. AT&T Manuals. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The M5 has two PMTs. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Enzyme dilution curves were performed for Lck, a tyrosine kinase withThe StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. S. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. The chamber. Figure 3. , Sigma cat. Sale. 00 +$250. Molecular Devices Corporation. com A. DRAWER button Opens and closes the microplate drawer when the instrument is in idle state. 1) EarlyTox Glutathione Assay (6. User Manuals, Guides and Specifications for your Molecular Devices SpectraMax M5 Laboratory Equipment. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. i3x. Published Dec 16, 2020 | Updated Dec 16, 2020. F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. This Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 1. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Description 1. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. item 6 Molecular Devices Spectramax M5 Multi-Mode Microplate Reader Parts/Repair Molecular Devices Spectramax M5 Multi-Mode Microplate Reader Parts/Repair $8,000. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. It is possible to search for the Overlord device driver for the instrument of your choice. Read More. SpectraMax M5 is ideal for laboratories requiring maximum flexibility in the detection of a. Softmax Pro GxP Software 6. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Turn on the microplate reader. Two sequential measurements should be carried out: at 620 nm for the cryptate emission, and at 665 nm for the specific signal emitted by the acceptor. Table1-1 SpectraMax ® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. , contact Molecular. Table 1. B Contact Us Phone: Japan (Osaka)+1-800-635-5577 Web: IP-One assay can be used to characterize compounds that act on Gq-coupled receptors in either adherent or suspension cells. For more advanced automation needs, Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. Molecular Devices Spectramax M5 Microplate Reader; Related products. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. i3. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Anne T. The ratioof the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation of Delta F (%)Molecular Devices SpectraMax M3 User Manual (86 pages) Multi-Mode Microplate Readers. For more information please visit us at: and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Unit also passed basic testing for: Shaking, Temp, Abs, FL, Cuv Capabilities: Fluorescence (top/bottom read) Luminescence (top/bottom read) Absorbance Time-ReSpectraMax® M4. The drawing shows a standard microplate of 15 mm height covered with a lid. Plate chamber design of the SpectraMax category 1 microplate readers. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Products : SpectraMax M2/M2e reader, FlexStation II (Obsolete), FlexStation 3 reader, SpectraMax M5/M5e reader. 1. OPTICSSpectraMax Glo and DuoLuc. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. D. Prepare your i3/i3x instrument for shipment. Spectranet customer portal. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug. 3. Table of Contents. 01 0. 2. Call 516-939-0888 to check current. temperature and fluorescence was measured at Ex/Em 490/585 nm using a Molecular Devices SpectraMax M5 microplate reader. es •. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Printed in USA. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Materials. Molecular Devices SoftMax ® Pro software was used to analyze and present the data. 2. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. 2), the fluorescence intensity of the FAM conjugated to the miR-148b mimic was measured in each well at 495/520 nm (Excitation/Emission) using a Molecular Devices Spectramax M5 Microplate/Cuvette Reader. 2. Published Dec 16, 2020 | Updated Dec 16, 2020. The cells were cultured in bulk flasks in DME + 10% FBS + 1% Pen/Strep/L-glutamine + 500 µg/mL of G418. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. #R7271) Akt1-PKBa 激酶 (Upstate Cat. Supplier: Molecular Devices. +1 800-635-5577. 9000-0756. Description 1. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. The following document is intended to demonstrate setup of this instrument. NORTH AMERICA. 1) EarlyTox NucView 488 Assay (6. SpectraMax Spectrofluorometers Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. This reader's dual monochromators for. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. Figure 2. With a wavelengthon the SpectraMax Multi-Mode Microp late Readers by issuing a single command in SoftMax ® Pro Software, Molecular Devices leading microplate data acquisition and analysis software platform. softmax pro software, spectramax paradigm, spectramax m5e/m5, walk-away automation, lab automation, fda 21 cfr oart 11, glp, gmp, stakmax Created Date: 1/11/2022 12:34:59. SpectraMax® M5 Microplate Reader Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. i3, M3, M4, M5, M5e, SpectraMax L, FlexStation 3 0200-6186: Cuvette Absorbance Validation Set SpectraMax. To purchase a newer software version,. The value for 1000 should be higher than the value for 900. on the SpectraMax Multi-Mode Microp late Readers by issuing a single command in SoftMax ® Pro Software, Molecular Devices leading microplate data acquisition and analysis software platform. The Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader and computer with preload software (StakMax Microplate Handling System/racks not included). SpectraMax® Microplate Readers and SoftMax® Pro Software. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. HTRF-compatible SpectraMax Multi-Mode Readers. The SpectraMax M2 and M2e are versatile and reliable. Detection modalities are shown in Table 1-1. -27. Manufacturer: Molecular Devices. . View regional contact information and Technical Support hours: Contact Us Hi Guest, welcome to. We would like to show you a description here but the site won’t allow us. 1. The chamber contains springs that position the cuvette in the proper alignment for a read. FI assay. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). This page. The built-in micro-volume sample port allows for working with sample volumes as small as 0. This assay kit is optimized for SpectraMax microplate readers from Molecular Devices, with a pre-configured protocol provided for customers using Molecular Devices’ SoftMax. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 3860 N First Street San Jose,CA 95134 USA +1 408. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. Check software compatibility with Windows 10 and locate your software installer. 1). To address this concern, Molecular Devices offers the FlexStation® 3 Multi-Mode Microplate Reader. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 4. 1. substrates using the SpectraMax M5 microplate reader and SoftMax® Pro Software. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 2. Description: Molecular Devices ’ unique SpectraDrop™ Micro-Volume Plate offers a high throughput and accurate solution for low volume measurement. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15. The Fluoroblok assays can be read on Spectramax M5/M5e, M4, M3, M2e, Gemini EM, Filtermax and Paradigm microplate readers. Learn about the SpectraMax® M5 Multi-Mode Microplate Reader, a versatile instrument for bioresearch and drug discovery applications. We therefore gathered salient. The system. • Tel. Home; Knowledge BaseMolecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. 5. Multimode Readers Comparison Chart. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. Table1-1 SpectraMax ® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5 e Absorbance X X X X SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 We would like to show you a description here but the site won’t allow us. Find regional support. Add to Cart. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. !Pathchecklm1000. Lower end of DNA standard curve. SpectraMax M 系列多功能微孔板读板机包括 M2/M2e、M3、M4、M5/M5e 等几款多功能酶标仪。. Table of Contents. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. FlexStation® 3 Multi-Mode Microplate Reader. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Results Luciferase emission spectrum. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. i3, M3, M4, M5, M5e, SpectraMax L, 6186 Luminescence FlexStation 3 Validation Plate 0200- Cuvette Absorbance SpectraMax Plus 384, ABS Plus, M2, M2e,. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 1. 5 - 2. for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax platform. 6% for the on-board optics (monochromators). The cells were cultured in bulk flasks in DME + 10% FBS + 1% Pen/Strep/L-glutamine + 500 µg/mL of G418. item 3 replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon. Modular Multi-Mode Reader also offers user upgradeability so that new detection capabilities can be added as users’ screening needs evolve. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. Histamine H1 is an endogenous receptor in HeLa cells. Various data transformation steps in the software are discussed, such as normalizing the GFP. -65. In the M2, M5 and Plus. The system provides automation for up to 50The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays. 7°C and the bottom to 36. Multimode Reader Comparison Chart. D 1 1. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. Molecular Devices, Inc. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Table of Contents. The drawing shows a standard microplate of 15 mm height covered with a lid. Cavicide (Warehouse, Cat # 79300360) 6. EUROPE. SpectraMax Instrument optical verification > IQ/OQ/PQ validation protocols for Molecular Devices microplate readers and washers > In-field reader Performance Certification. Sunnyvale, California -- October 5, 2017. Bookings are free, but you will need to use iLab to book. Plate Formats: 6, 12, 24, 48, 96, 384 Wells Plates. There should be a value for 900 and 1000. SpectraMax® ABS Plus. This document is provided to customers who have purchased Molecular Devices, Inc. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Step 1: Open the SoftMax Pro IMAP FP protocol. 5. View the Molecular Devices websiteThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seWe would like to show you a description here but the site won’t allow us. OPTICSon the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET assays. SpectraMax Injector Cartridge with SmartInject™ Technology. Conventional Electrophysiology. Compatible with SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, FlexStation 3 readers 0200-6191: Gray replacement validation plate case Compatible with. Stray light. Spectranet customer portalMolecular Devices provides a means of validating the optical performance of the SpectraMax M2, SpectraMax M5, PLUS, PLUS 384, 190, 340PC, 340PC 384 micro-plate spectrophotometers and the VersaMax microplate reader. Pleasanton, CA, USA. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. Description 1. EUROPE. -49. This problem has been remedied by the introduction of Molecular Devices PathCheck Technology, which enables determination of the pathlength in each well of a microplate and automatically normalizes the absorbance value to a 1-cm pathlength. View the Molecular Devices website. Finding your SpectraMax® Paradigm and DTX instrument serial number, MultiMode Software version, and Operating System (OS). #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. Molecular. Figure 3. This manual is subject to change without notice. (“Molecular Devices”) equipment, software, reagents, and consumables to use in theWe would like to show you a description here but the site won’t allow us. High-resolution touchscreen allows for quick set up of methods. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. Photometric accuracy. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1.